Asparagine-linked protein glycosylation: from eukaryotic to prokaryotic systems.

Investigating Asparagine-Linked Protein Glycosylation in Eukaryotic and Prokaryotic Systems

Complex glycosylation of Skp1 in Dictyostelium: implications for the modification of other eukaryotic cytoplasmic and nuclear proteins.

Recently, complex O-glycosylation of the cytoplasmic/nuclear protein Skp1 has been characterized in the eukaryotic microorganism Dictyostelium. Skp1's glycosylation is mediated by the sequential action of a prolyl hydroxylase and five conventional sugar nucleotide-dependent glycosyltransferase activities that reside in the cytoplasm rather than the secretory compartment. The Skp1-HyPro GlcNAcTr...

Prediction of Glycosylation Across the Human Proteome and the Correlation to Protein Function

The addition of a carbohydrate moeity to the side chain of a residue in a protein chain in uences the physicochemical properties of the protein Gly cosylation is known to alter proteolytic resistance protein solubility stability local structure lifetime in circulation and immunogenicity Of the various forms of protein glycosylation found in eukaryotic systems the most important types are N link...

تهیه سازه‌های مناسب به‌منظور بیان ژن اینترفرون گامای انسانی در Leishmania tarentolae

 Background and Objective: The aim of the present study was to express recombinant human interferon-gamma in Leishmania tarentolae. The Leishmania expression system represents the combination of easy handling known from bacterial expression systems with the potential of a eukaryotic protein expression, folding and modification system. The trypanosomatid protozoan host Leishmania tarent...

An evolving view of the eukaryotic oligosaccharyltransferase.

Asparagine-linked glycosylation (ALG) is one of the most common protein modification reactions in eukaryotic cells, as many proteins that are translocated across or integrated into the rough endoplasmic reticulum (RER) carry N-linked oligosaccharides. Although the primary focus of this review will be the structure and function of the eukaryotic oligosaccharyltransferase (OST), key findings prov...